Isotype Controls
Isotype Control is a class of antibody that is used as a negative control in antibody staining applications.
Features-Isotype Control have the same immunoglobulin class and subclass as the primary antibody which is used in the experiment but lack affinity to the protein of interest.
Benefits-Isotype Control makes experiments credible.
Antibodies bind to a specific protein or antigen, but sometimes an antibody can bind to other proteins or cellular components in a non-specific manner. Experimental controls are critical in determining if the antibodies used in your experiment have any off-target binding capabilities. Off-target or non-specific binding by antibodies lead to false positive results, making it difficult to determine background staining from antigen-specific staining. Therefore, it is important to always include experimental controls to ensure reliable results.
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